Although the majority of the genetic background in a ril is fi xed, a small percentage of loci will be segregating. From these 383 lines, a population of 200 different multiparental recombinant inbred lines mprils was randomly picked for mrna sequencing to obtain the genetic variation in the coding sequence. Development of chromosomal segment substitution lines. Pdf to locate map genes a populatton segregating for such genes is required. An ril is formed by crossing two inbred strains followed by repeated selfing or sibling mating to create a new inbred line whose genome is a mosaic of the parental genomes figure 1. Recombinant inbred lines were generated from the cross of nm24016 with tm1, made in 1994. Genetic variation for agronomic and fiber properties in an introgressed recombinant inbred population of cotton. The 32 linkages groups included 882 single nucleotide.
Inbred lines article about inbred lines by the free. Genetic variation for agronomic characters and drought. Although a handful of isolated recombinant inbred ri mouse strains were developed during the. Pdf recombinant inbred lines rils are a collection of strains that can be used to map quantitative trait loci. The bulked f8 seeds we are distributing from the first 100 lines were supplied to us by the nottingham arabidopsis stock centre. Recombinant inbred lines for genetic mapping in tomato. Pdf design and construction of recombinant inbred lines. Recombinant inbred lines derived from potato interspecific. We report the genomic structures of irils from the ibm. Mp8, nsl 520824 map recombinant inbred line mapping population was developed through single seed descent from the f 2 generation to the f 5 generation. We simplifi ed schnells intricate fi gure to the four basic regressions across time for each of the three studies. Intermated recombinant inbred lines irils in plants, or advanced recombinant inbred strains in animals, are constructed by carrying out. Inbred the offspring of a crosspollinating plant obtained as a result of repeated forced selfpollination.
To enable crossovers on chromosome x and to generate extra crossovers, the heterozygous f2 obtained from. To rule out experimental error such as mislabeling of samples or files, we repeated sample preparation and array genotyping. Input file format for the windows version of irilmap can be either the commonlyused mapmakerlike output format or tabulated files. Inbred strains also called inbred lines, or rarely for animals linear animals are individuals of a particular species which are nearly identical to each other in genotype due to long inbreeding. Joint analysis of nearisogenic and recombinant inbred. Use of recombinant inbred lines rils to identify, locate and map. The inbred lines from vniimk, krasnodar russia used in this study as donors for oil quality, i. Among the new inbred lines related to b73, line 2602772 distinguished itself by a high potential for grain yield when crossed with mo17. Responses of recombinant inbred lines of cowpea vigna unguiculata l walp. Conception rate at the first oestrus at which the ewe was mated in each year declined almost linearly from 071 f 2 generation.
However, getting the inbred lines in the first place can be a hassle. Recombinant inbred line rils population development in. The collaborative cross cc is a large panel of recently established multiparental recombinant inbred mouse lines specifically designed to overcome. Progress toward the development of recombinant inbred lines. Nonparental banding patterns in recombinant inbred line. Genetic linkage map of a recombinant inbred lines in tomato. Intermated recombinant inbred lines listed as iril. A ril that is heterozygous at the qtl can be selfed and a set of hifs, lines diff ering for alleles at that qtl with an otherwise. Rnaseq using bulked recombinant inbred line populations. Wed like to understand how you use our websites in order to improve them. The grain yield potential of test crosses with mo17 inbred tester, as well as ear length, number of grain rows per ear and 1,000grain mass of lines perse were tested. Intermated recombinant inbred lines how is intermated. Recombinant inbred lines for genetic mapping in tomato springerlink. Pdf the use of recombinant inbred lines rils for genetic.
With repeated selfpollination the viability and yield of the offspring are decreased, while the. Inbred lines definition of inbred lines by medical. Combining ability of maize inbred lines evaluated in three. Effects of rapid inbreeding and of crossing of inbred. Introgression lines are created by backcrossing an f1 of a cross between two parental lines to. A recombinant inbred strain or recombinant inbred line is an organism with chromosomes that incorporate an essentially permanent set of recombination events between chromosomes inherited from two or more inbred strains.
Ril is defined as recombinant inbred lines frequently. Rils were arranged in randomized complete block design with three replications per location. Broman1 department of biostatistics, johns hopkins university, baltimore, maryland 21205 manuscript received august 20, 2004 accepted for publication november 5, 2004 abstract recombinant inbred lines. A highresolution map of arabidopsis recombinant inbred lines by. Intermated recombinant inbred lines how is intermated recombinant inbred lines. Evidence is presented that transformation frequency for inbred lines. A multiparent recombinant inbred line population of c. Conference paper pdf available april 20 with 7 reads how we.
However, the number of potential crosses grows rapidly as more and more inbred lines. Construction of epigenetic recombinant inbred lines epiril. The use of recombinant inbred lines rils for genetic mapping article pdf available in methods in molecular biology 82. The genetic stability of partially inbred lines of maize.
Progress 100109 to 093010 outputs progress report objectives from ad416 develop inbred lines and hybrids adapted to the abiotic and biotic stresses characteristic of the midatlantic region and. Inbreeding is the production of offspring from the mating or breeding of individuals or organisms that are closely related genetically. As each ril is an inbred strain, and so can be propagated eternally, a panel of rils has a number of advantages for genetic mapping. Breeding designs for recombinant inbred advanced intercross lines. Broman1 department of biostatistics, johns hopkins university, baltimore, maryland 21205 manuscript received august 20, 2004 accepted for publication november 5, 2004 abstract recombinant inbred lines rils can serve as powerful tools for genetic mapping. Reduce individual variation by phenotyping multiple individuals from.
Genetic dissection of intermated recombinant inbred lines. Development of cssl from a backcross recombinant inbred population of interspecific rice cross 17 the number of xb homozygotes, n 2 the heterozygotes, and n the total number of informative individuals. Cucumber recombinant inbred lines cucurbit breeding. Ones available have been based on a dominant selffertility gene or allele. Improved agrobacteriummediated transformation of three. The afrc funded the commercial bulking of these lines. A subset of three maize inbred lines was then systematically evaluated for postinfection ecif and tf on four media regimes. Columbia recombinant inbred lines rils of arabidopsis have been used in order to identify and localize chromosome regions invo. However, some studies have suggested the potential use of partially inbred lines. Pdf responses of recombinant inbred lines of cowpea. An ril is formed by crossing two inbred strains followed by repeated selfing or sibling mating to create a new inbred line whose genome is a mosaic of the parental genomes.
Recombinant inbred lines derived from cultivars of pea for understanding the genetic basis of variation in breeders traits volume 16 special issue carol moreau, maggie knox, lynda turner, tracey rayner, jane thomas, haidee philpott, steve belcher, keith fox, noel ellis, claire domoney. Recombinant inbred lines derived from cultivars of pea for. A cross between the cultivated tomato lycopersicon esculentum and a related wild species l. Comparison of introgression a and recombinant inbred lines b. Analyses were carried out both for the data overall and within inbred lines. Recombinant inbred lines derived from an advanced intercross, in which multiple generations of mating have increased the density of recombination. Combining ability of maize inbred lines evaluated in three environments in brazil. Allelic variation at these loci was measured in a recombinant inbred line population, which defined the location of 815 recombination breakpoints. Registration of the louisealpowa wheat recombinant inbred.
N6 or ms salts in each of two distinct media backgrounds. A recombinant inbred line population of tomato and its genetic map 443 derived from a cross between two inbred lines via selfbreeding and singleseed descent breeding approach until homozygosity or. Ho and ar were less in the line 1 hereford inbred cattle population than in the parent hereford breed. Eleven maize inbred lines were prescreened for transformation using n6 salts. Recombinant inbred lines rils can serve as powerful tools for genetic mapping. By analogy, the term is used in human reproduction, but more commonly. Inbred lines definition of inbred lines by the free. Design and construction of recombinant inbred lines.
Molecular analysis samples of the molecular analysis to observe the genetic distance among inbred lines used. Dm f1 hybrid m6 f2 population 211 clones selfpollinate. The genomes of recombinant inbred lines complex trait. Creating hybrids in beta is problematic since flowers are slightly protandrous. All data generated or analyzed during this study are included in this published article and its supplementary information files.
The c and mathematica codes for these different tasks are provided in file s2. Distribution of parental genome blocks in recombinant inbred lines. Construction of epigenetic recombinant inbred lines ep. Performance prediction of f hybrids between recombinant. A common strategy to create highyielding plants is hybrid breeding. Comparison of introgression a and recombinant inbred. Genetic variation and differentiation in parentdescendant. F1 and f2 generations are produced by intercrossing the inbred strains. Recombinant inbred lines how is recombinant inbred lines. Lines showing 90% or higher xb proportions were selected. We consider recombinant inbred lines obtained by crossing two given. To create homozygous genotypes, single worms were selected from the f2 as well as from the f2 intercross for 6 generations of singleworm inbreeding. Our goal was to identify lines resistant to both fungi. Evaluation of maize inbred lines for resistance to.
63 1166 454 837 1440 1061 319 601 86 1401 811 1519 343 1068 577 1334 220 874 229 897 1199 229 814 1366 401 951 910 553 379 1034 1219 835 955 510 627 40 1367 1159 7 1419 961 409 1270 48 717 689 426 1394 118